Composite

Part:BBa_K646008

Designed by: David Dubow   Group: iGEM11_Macquarie_Australia   (2011-09-30)


T7 promoter with Heme oxygenase and Agrobacterium tumefaciens bacteriophytochrome

This is an assembly of an Rhizobium radiobacter/Agrobacterium tumefaciens isolated bacteriophytochrome gene with a Synechocytis heme oxygenase 1 gene under a T7 promoter. The bacteriophytochrome gene produces a protein that interacts with Biliverdin, a chromophore. The chromophore is produced by heme oxygenase oxidation of heme in the heme metabolic pathway to produce the biliverdin. Sequence contains a Ribosome Binding Site. Sequence from National Center for Biotechnology.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2650
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1979
    Illegal NgoMIV site found at 2477
    Illegal AgeI site found at 2542
  • 1000
    COMPATIBLE WITH RFC[1000]


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