Composite
Part:BBa_K646008
Designed by: David Dubow Group: iGEM11_Macquarie_Australia (2011-09-30)
T7 promoter with Heme oxygenase and Agrobacterium tumefaciens bacteriophytochrome
This is an assembly of an Rhizobium radiobacter/Agrobacterium tumefaciens isolated bacteriophytochrome gene with a Synechocytis heme oxygenase 1 gene under a T7 promoter. The bacteriophytochrome gene produces a protein that interacts with Biliverdin, a chromophore. The chromophore is produced by heme oxygenase oxidation of heme in the heme metabolic pathway to produce the biliverdin. Sequence contains a Ribosome Binding Site. Sequence from National Center for Biotechnology.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2650
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1979
Illegal NgoMIV site found at 2477
Illegal AgeI site found at 2542 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |